Part:BBa_K563054:Design

Vector pYD, and can transfer into nucles of S.cerevisivae

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 936
  Illegal XbaI site found at 975
  Illegal XbaI site found at 2019
  Illegal PstI site found at 801
  Illegal PstI site found at 941
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 936
  Illegal NheI site found at 858
  Illegal PstI site found at 801
  Illegal PstI site found at 941
  Illegal NotI site found at 962
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 936
  Illegal BamHI site found at 921
  Illegal XhoI site found at 969
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 936
  Illegal XbaI site found at 975
  Illegal XbaI site found at 2019
  Illegal PstI site found at 801
  Illegal PstI site found at 941
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 936
  Illegal XbaI site found at 975
  Illegal XbaI site found at 2019
  Illegal PstI site found at 801
  Illegal PstI site found at 941
  Illegal AgeI site found at 71
  Illegal AgeI site found at 1038
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1020
  Illegal BsaI site found at 3646
  Illegal SapI.rc site found at 2624
  Illegal SapI.rc site found at 4728
  

Design Notes

In our project, to make our mutation of Tor2 easier, we separate complete Tor2 into three parts and mutate them independently. So we reconstruct pYD vector to add certain restriction sites for linking mutant Tor2 and original Tor2

Source

AGA2, GAL1, TRP5 and CEN come from S.cerevisivae. Amppicillin resistance gene and the pUC origin for selection and replication come from E.coli